Plasmid_Backbone

Part:BBa_K4675009:Design

Designed by: Julia Uhlenbrock   Group: iGEM23_UniMuenster   (2023-10-04)


pSB3CY_Zeo shuttle vector backbone for Komagataella phaffii


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4376
    Illegal SpeI site found at 2622
    Illegal PstI site found at 1105
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4376
    Illegal NheI site found at 3062
    Illegal SpeI site found at 2622
    Illegal PstI site found at 1105
    Illegal NotI site found at 3003
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4376
    Illegal BglII site found at 2518
    Illegal BamHI site found at 2441
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4376
    Illegal SpeI site found at 2622
    Illegal PstI site found at 1105
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4376
    Illegal SpeI site found at 2622
    Illegal PstI site found at 1105
    Illegal AgeI site found at 793
    Illegal AgeI site found at 905
    Illegal AgeI site found at 2083
    Illegal AgeI site found at 2406
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1378
    Illegal BsaI site found at 4382
    Illegal BsaI.rc site found at 1099
    Illegal SapI site found at 1082
    Illegal SapI.rc site found at 6


Design Notes

.


Source

synthesized

References